Description

NAD+ (Nicotinamide Adenine Dinucleotide) is an essential oxidized coenzyme found in all living cells. It serves as a critical electron carrier in redox reactions and acts as a necessary substrate for enzymes that regulate cellular homeostasis, including sirtuins and poly-ADP-ribose polymerases (PARPs). In the landscape of longevity research, NAD+ is the primary entity studied for its role in reversing mitochondrial decay and maintaining genomic integrity.

Performance Peptides Canada provides NAD+ in 1000mg vials at 99%+ purity. Every batch is HPLC-verified to ensure the absence of degradation products, providing a stable foundation for research into cellular metabolism and age-related biochemical decline.

Technical Specifications & Chemical Profile

Our NAD+ is synthesized to meet the following analytical benchmarks:

• Molecular Formula: C21,H27,N7,O14,P2
• Molecular Weight: 663.43 g/mol
• CAS Number: 53-84-9
• Purity: ≥99.0% (Verified via HPLC/MS)
• Physical Form: Lyophilized (Freeze-Dried) white powder

Primary Research Mechanisms & Mitochondrial Flux

NAD+ research is centered on its dual role as a metabolic cofactor and a signaling nucleotide.

1. The NAD+/NADH Ratio & ATP Production

NAD+ is the primary hydride acceptor in the Tricarboxylic Acid (TCA) Cycle and glycolysis. It accepts electrons to become NADH, which then donates them to the Electron Transport Chain (ETC) to drive ATP synthesis. Researchers utilize the 1000mg format to study how fluctuating NAD+ levels influence mitochondrial bioenergetics and “metabolic flexibility” in aging models.

2. Sirtuin Activation (The Longevity Pathway)

NAD+ is the required “fuel” for Sirtuins (SIRT1-SIRT7), a family of protein deacetylases that regulate gene expression and DNA repair. As NAD+ levels naturally decline with age, the activity of sirtuins decreases. Research in this category focuses on how replenishing NAD+ pools can reactivate these pathways to protect against cellular senescence.

3. PARP-Mediated DNA Repair

When DNA damage occurs, the enzyme PARP-1 is activated to initiate repair. PARP-1 consumes significant amounts of NAD+ to function. Researchers study NAD+ to understand the “NAD+ competition” between DNA repair mechanisms and energy production, which is a hallmark of cellular stress research.

Reconstitution & Handling Protocols

NAD+ is a highly hygroscopic (moisture-absorbing) compound and is more sensitive to environmental factors than many standard peptides:

• Reconstitution: Recommended diluent is Sterile Water for Injection or 0.9% Sodium Chloride.
• Technique: Gently introduce the diluent. The powder should dissolve almost instantly. Do not shake.
• Cold Chain Importance: NAD+ is particularly sensitive to thermal degradation. It is vital to keep the vial refrigerated at all times after reconstitution.

Stability & Storage Data

Note: For maximal research accuracy, it is highly recommended to use NAD+ within 5 days of reconstitution, as it can begin to hydrolyze into nicotinamide and ADP-ribose if left in solution for extended periods.

NAD+ Research FAQ

Q: What is the difference between NAD+ and NADH?

A: NAD+ is the oxidized form (the electron acceptor), while NADH is the reduced form (the electron donor). Research typically focuses on NAD+ because its levels decline with age and it is the form required for sirtuin activation.

Q: Why is your NAD+ 1000mg, whereas peptides are usually 10mg?

A: Research protocols for NAD+ generally require significantly higher molar concentrations than signaling peptides. Our 1000mg format allows for the high-dose investigations necessary for mitochondrial and redox research.

Q: Is this shipped domestically in Canada?

A: Yes. Performance Peptides Canada ships all NAD+ orders from our domestic facilities. Because NAD+ is sensitive to heat, our fast 1-3 day Canadian shipping is essential to preserve the coenzyme’s integrity.