Description

Bacteriostatic Water for Injection USP 30mL

Bacteriostatic Water for Injection USP is a sterile, non-pyrogenic preparation of water for injection containing 0.9% benzyl alcohol as an antimicrobial preservative. Registered with Health Canada under DIN 000802022, it meets United States Pharmacopeia (USP) standards for sterility, endotoxin limits, and pH the same standards applied to pharmaceutical-grade injectable preparations.

It is the diluent of choice for reconstituting lyophilized peptides and other compounds in research and laboratory settings, providing a stable, multi-dose solution that resists bacterial contamination between uses.

Product Specifications

Specification	Detail
Volume	30mL per vial
Preservative	0.9% Benzyl Alcohol (w/v)
Standard	United States Pharmacopeia (USP)
Sterility	Non-pyrogenic, sterile
DIN	000802022
Format	Multi-dose vial
pH	4.5 to 7.0

Why Bacteriostatic Water for Peptide Reconstitution

When reconstituting lyophilized peptide compounds for laboratory use, diluent selection directly affects both the stability of the reconstituted solution and the viable research window after reconstitution.

Benzyl alcohol as a bacteriostatic preservative inhibits the growth of bacteria introduced during repeated needle insertions into a multi-dose vial a critical property when a single vial will be accessed multiple times over an extended research period. Sterile water without a preservative does not provide this protection and should only be used for single-dose applications.

Compared to sterile saline (0.9% sodium chloride), bacteriostatic water is the preferred diluent for most lyophilized peptides because the absence of sodium chloride reduces the risk of aggregation in compounds sensitive to ionic strength. For peptides where solubility is challenging, the slightly acidic pH range of bacteriostatic water (4.5 to 7.0) can also assist initial dissolution.

Reconstitution Guidelines

The following protocol applies when using bacteriostatic water to reconstitute lyophilized research compounds:

• Allow the bacteriostatic water vial to reach room temperature before use
• Clean the vial septum with an alcohol swab and allow to dry before inserting a needle
• Draw the required volume of bacteriostatic water slowly into a sterile syringe
• Introduce the diluent into the lyophilized vial slowly along the interior wall do not inject directly onto the lyophilized cake
• Swirl the vial gently until fully dissolved do not shake or vortex
• Once reconstituted, store the peptide vial at 2°C to 8°C and use within the compound’s recommended reconstituted stability window

Storage

Condition	Detail
Unopened	Store at room temperature (15°C to 30°C), away from direct light
After first use	Store at room temperature or refrigerated (2°C to 8°C)
Do not freeze	Freezing may compromise vial integrity and sterility

Important Safety Information

• Not for use in newborns. Benzyl alcohol has been associated with toxicity in neonates. This product must not be used as a diluent in preparations intended for newborn patients.
• This product is registered with Health Canada under DIN 000802022 and meets USP standards for injectable preparations.
• Single-use needles and syringes should be used for each withdrawal from the vial to maintain sterility.
• Inspect the solution before each use do not use if the solution appears cloudy, contains particulates, or if the vial seal has been compromised.

Bacteriostatic Water FAQ

Q: What is the difference between bacteriostatic water and sterile water for injection?

A: Sterile water for injection contains no preservative and is intended for single-dose use only. Bacteriostatic water contains 0.9% benzyl alcohol, which inhibits bacterial growth and allows the vial to be accessed multiple times safely over an extended period. For research applications requiring repeated withdrawals from a single vial, bacteriostatic water is the appropriate choice.

Q: Can bacteriostatic water be used to reconstitute all lyophilized peptides?

A: Bacteriostatic water is compatible with the majority of lyophilized research peptides. For compounds with specific pH sensitivity or known incompatibility with benzyl alcohol, the compound’s technical documentation should be consulted before selecting a diluent. When in doubt, sterile saline (0.9% sodium chloride) is an alternative for single-use reconstitutions.

Q: How long does a reconstituted peptide remain stable when diluted with bacteriostatic water?

A: Reconstituted stability varies by compound. As a general guideline, most lyophilized peptides reconstituted with bacteriostatic water remain stable for 7 to 14 days when stored at 2°C to 8°C. Individual compound stability data should be referenced for precise windows.

Q: Is this product Health Canada approved?

A: Yes. This Bacteriostatic Water for Injection USP is registered with Health Canada under Drug Identification Number (DIN) 000802022, confirming it meets Canadian regulatory standards for sterile injectable pharmaceutical preparations.

Q: Where does this product ship from?

A: This product is fulfilled domestically from within Canada.