Description

TB-500 10mg is available at Performance Peptides Canada for qualified Canadian researchers. Each batch is HPLC-verified at 99%+ purity with a Certificate of Analysis included with every order. TB-500 is a synthetic peptide fragment derived from Thymosin Beta-4 (Tβ4), a naturally occurring 43-amino-acid protein. TB-500 isolates the actin-binding domain of Tβ4 — the seven-amino-acid sequence Ac-LKKTETQ (residues 17 to 23) — studied in non-clinical laboratory research for its role in actin sequestration, cell migration, angiogenesis, and tissue regeneration signalling. With a molecular weight of approximately 889 Da, TB-500 is structurally distinct from the full Thymosin Beta-4 protein and is not interchangeable with it in all research protocols. All products sold by Performance Peptides Canada are strictly for laboratory and research use only and are not intended for human consumption, medical use, or diagnostic applications.

TB-500 is a synthetic heptapeptide (7-amino acid sequence) that represents the N-acetylated active region of the naturally occurring protein, Thymosin Beta-4 (Tβ4). Specifically corresponding to the amino acids 17–23 of the full Tβ4 molecule, TB-500 is the primary entity studied for its ability to sequester G-actin and facilitate rapid cellular motility.

Performance Peptides Canada provides 10mg TB-500 vials with 99%+ HPLC-verified purity. Our domestic Canadian fulfillment ensures that this sensitive regenerative ligand is handled with strict climate controls to preserve its actin-binding efficiency.

Technical Specifications & Chemical Architecture

For researchers requiring molecular precision, our TB-500 is synthesized to the following analytical standards:

• Molecular Formula: C₃₈H₆₈N₁₀O₁₄
• Molecular Weight: 889.01 g/mol
• Amino Acid Sequence: Ac-Leu-Lys-Lys-Thr-Glu-Thr-Gln (Ac-LKKTETQ)
• CAS Number: 885340-08-9
• Purity: ≥99.0% (Verified via HPLC/MS)
• Structure: Synthetic N-terminal acetylated fragment.

Primary Research Mechanisms & Action

Unlike full-length proteins, the TB-500 fragment is studied for its unique systemic bioavailability and its specific role in the actin-sequestering pathway.

1. G-Actin Sequestration & Cytoskeletal Remodeling

TB-500’s primary mechanism of action involves binding to G-actin (globular actin) monomers in a 1:1 ratio. By preventing the polymerization of G-actin into F-actin (filamentous actin), TB-500 maintains a pool of monomeric actin essential for rapid cytoskeletal remodeling. This allows cells to change shape and migrate more efficiently toward injury sites in experimental models.

2. Angiogenesis & Endothelial Migration

In vascular research, TB-500 is utilized to study the upregulation of Vascular Endothelial Growth Factor (VEGF). It promotes the migration of endothelial cells and the formation of new capillary networks, a process essential for restoring oxygen and nutrient delivery to ischemic or damaged tissues.

3. Anti-Fibrotic & Anti-Inflammatory Signaling

Research indicates that TB-500 may reduce the presence of myofibroblasts at wound sites, leading to decreased scar tissue formation (fibrosis). Furthermore, it is studied for its ability to suppress pro-inflammatory cytokines such as TNF-α and IL-1β, fostering a cellular environment conducive to organized tissue regeneration.

Storage & Reconstitution Protocol

Peptide integrity is highly dependent on laboratory handling.

• Reconstitution: We recommend using Bacteriostatic Water or 0.9% Sterile Saline.
• Method: Slowly introduce 2–3mL of diluent down the glass wall. Gently swirl—do not shake—as mechanical stress can disrupt the delicate peptide bonds.
• Temperature Sensitivity: Allow the lyophilized vial to reach room temperature before reconstitution to prevent moisture condensation (hydrolysis).

Stability Benchmarks

Note: Once reconstituted, do not freeze the solution, as this can lead to peptide denaturation.

TB-500 Canada, Research & Compliance FAQ

Q: Is TB-500 legal to buy in Canada?

A: TB-500 is legal to purchase in Canada for legitimate laboratory research purposes. It is not a controlled substance under the Controlled Drugs and Substances Act (Canada). However, it is not approved by Health Canada for human consumption or therapeutic use. Performance Peptides Canada supplies TB-500 exclusively for non-clinical laboratory research use in compliance with Canadian regulations.

Q: What does the TB-500 peptide do in research models?

A: TB-500 (Thymosin Beta-4 fragment, sequence Ac-LKKTETQ) is studied for its role in cellular migration, tissue repair, and anti-inflammatory signalling. Research models examine its effects on actin regulation, wound healing, tendon and ligament repair mechanisms, and angiogenesis. It is one of the most widely studied synthetic peptide fragments in regenerative research.

Q: Are peptides available in Canada for research?

A: Yes. Research-grade peptides including TB-500 are legally available in Canada for laboratory and scientific research purposes. Performance Peptides Canada fulfills all orders domestically, ensuring HPLC-verified purity standards and cold-chain integrity throughout delivery. All products are supplied for research use only and are not approved for human therapeutic use.

Q: Is TB-500 safe to handle in a research setting?

A: TB-500 is a synthetic peptide fragment handled under standard laboratory protocols. As with all research compounds, appropriate safety procedures should be followed during reconstitution and handling. Performance Peptides Canada provides a Certificate of Analysis (COA) with every batch confirming purity and identity. All products are for research purposes only and are not intended for human or veterinary use.

TB-500 Product & Sourcing FAQ

Q: What is the difference between TB-500 and Thymosin Beta-4?

A: In a laboratory context, Thymosin Beta-4 refers to the full 43-amino acid protein (MW approximately 4,963 Da), whereas TB-500 specifically refers to the synthetic 7-amino acid active fragment (MW approximately 889 Da). The TB-500 fragment (sequence: Ac-LKKTETQ) is often preferred in research for its stability, smaller molecular footprint, and targeted action on cellular migration and tissue repair.

Q: Can TB-500 be used with other peptides?

A: Many researchers study TB-500 in conjunction with BPC-157 to evaluate the synergy between TB-500’s systemic cellular migration effects and BPC-157’s localized connective tissue repair pathways.

Q: Is your TB-500 shipped from Canada?

A: Yes. Performance Peptides Canada ships all orders from domestic facilities to ensure rapid delivery and eliminate the degradation risks associated with international customs delays.

Q: Is TB-500 legal to purchase in Canada?

A: TB-500 is sold in Canada strictly as a research reagent for qualified laboratory and non-clinical research use. It is not approved by Health Canada for human use and is not a licensed drug. TB-500 has been on the World Anti-Doping Agency (WADA) Prohibited List since 2011 under category S0 as a non-approved substance, restricting its use by competitive athletes. All orders from Performance Peptides Canada are supplied under a research-use-only framework and are not intended for consumption or therapeutic application.

Q: What purity standard does Performance Peptides Canada use for TB-500?

A: Every batch of TB-500 10mg supplied by Performance Peptides Canada is HPLC-verified at 99%+ purity. A batch-specific Certificate of Analysis is included with every order, allowing Canadian researchers to independently verify compound identity, sequence integrity, and purity data before laboratory use.

Q: How is TB-500 shipped within Canada?

A: TB-500 orders are fulfilled domestically from within Canada, eliminating customs delays and cross-border handling. Orders ship as lyophilised powder in sealed research-grade vials. Domestic fulfillment ensures faster delivery and removes the risk of temperature excursions or customs holds associated with international peptide shipments.